Typical users of computerized stereology system such as Stereologer® include students and bioscientists in academia centers, government labs and pharmaceutical companies. Figure 1: Stereology Parameters visualized, allow for unbiased methods Applications for Stereology Systems This application is made through stereology systems, which are made up of stereology software interfaced with peripheral microscope hardware. When bias is present, the results converge on values some distance from the true value. Eliminating or avoiding all known sources of bias leads to theoretically unbiased estimates, i.e., results that converge on their expected or true values as tissue sampling continues. Modern stereology is typically applied to studies involving stained cells where structural changes such as cell loss, synaptic degeneration, loss of volume (atrophy) is quantified. This allows for quantification of structures in an unbiased (aka design-based), rather than assumption- or model-based approaches. Stereology uses unbiased sampling and estimation methods to quantify tissue with first-order stereological parameters such as – volume (V), surface area (S), length (L), and number (N) as seen in Figure 1. Incorporating Unbiased Stereology into Your Research
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